Detailed information of TA system TA156744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2022150..2022751
• Replicon: chromosome
• Accession: NZ_CP053383
• Organism: Halomonas sulfidivorans strain MCCC 1A13718

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HNO53_RS09410
• Protein ID: WP_209478082.1
• Coordinates: 2022150..2022440 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HNO53_RS09415
• Protein ID: WP_209478083.1
• Coordinates: 2022446..2022751 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNO53_RS09390 (HNO53_09390)	2018183..2018890	-	708	WP_209478075.1	lipopolysaccharide kinase InaA family protein	-
HNO53_RS09395 (HNO53_09395)	2018903..2019961	-	1059	WP_209478076.1	glycosyltransferase	-
HNO53_RS09400 (HNO53_09400)	2020523..2021317	+	795	WP_242597597.1	glycosyltransferase	-
HNO53_RS09405 (HNO53_09405)	2021373..2021897	-	525	WP_209478080.1	DUF1269 domain-containing protein	-
HNO53_RS09410 (HNO53_09410)	2022150..2022440	+	291	WP_209478082.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HNO53_RS09415 (HNO53_09415)	2022446..2022751	+	306	WP_209478083.1	HigA family addiction module antitoxin	Antitoxin
HNO53_RS09420 (HNO53_09420)	2022778..2023557	-	780	WP_209478085.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
HNO53_RS09425 (HNO53_09425)	2023607..2023936	-	330	WP_209478087.1	helix-turn-helix domain-containing protein	-
HNO53_RS09430 (HNO53_09430)	2024455..2025735	-	1281	WP_209478089.1	hemolysin family protein	-
HNO53_RS21595	2025738..2025869	-	132	WP_275951366.1	hypothetical protein	-
HNO53_RS09435 (HNO53_09440)	2025912..2026922	-	1011	WP_209478091.1	GSU2403 family nucleotidyltransferase fold protein	-
HNO53_RS09440 (HNO53_09445)	2027034..2027273	-	240	WP_242597598.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1992006..2022751	30745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-69)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11257.68 Da
• Isoelectric Point: 6.4820

>T156744 WP_209478082.1 NZ_CP053383:2022150-2022440 [Halomonas sulfidivorans]
MIDSFRDAWLEAYFVDGIAHRRIPNAIESALTRKLDIIEAAAEENDLRVPPGNRFEHLKGQLEGKCSIRVNKQYRLIFEW
DGERAHNVYLDSHTYR

Nucleotide

• Length: 291 bp

>T156744 NZ_CP069070:c1577286-1576885 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGTGGGCAT
GA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11670.57 Da
• Isoelectric Point: 8.5516

>AT156744 WP_209478083.1 NZ_CP053383:2022446-2022751 [Halomonas sulfidivorans]
MFKINRKPTTVGEMLTHEFLEPFDLSQTKLAERMGVSPRLVNELCRNKRAVTADTALMLSRVFGTSPQFWLNLQQKVDLW
EAVHDAKRLERIEKAQPLECA

Nucleotide

• Length: 306 bp

>AT156744 NZ_CP069070:c1577540-1577283 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA