Detailed information of TA system TA156709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 118574..119231
• Replicon: plasmid p1
• Accession: NZ_CP053365
• Organism: Klebsiella pneumoniae strain BA2275

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: C5707_RS27950
• Protein ID: WP_000270043.1
• Coordinates: 118881..119231 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5707_RS27945
• Protein ID: WP_000124640.1
• Coordinates: 118574..118876 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5707_RS27895	114208..114636	+	429	WP_000591076.1	hypothetical protein	-
C5707_RS27900	114694..115053	+	360	WP_000422769.1	hypothetical protein	-
C5707_RS27905	115053..115499	+	447	WP_000919343.1	hypothetical protein	-
C5707_RS27910	115496..116014	+	519	WP_000210757.1	nitrite reductase	-
C5707_RS27915	116014..116244	+	231	WP_000972665.1	hypothetical protein	-
C5707_RS27920	116231..117088	+	858	WP_001167036.1	hypothetical protein	-
C5707_RS27925	117000..117305	+	306	WP_171820680.1	hypothetical protein	-
C5707_RS27930	117308..117835	+	528	WP_004201083.1	thermonuclease family protein	-
C5707_RS27935	117893..118165	+	273	WP_001043046.1	HU family DNA-binding protein	-
C5707_RS27940	118254..118547	+	294	WP_001239998.1	hypothetical protein	-
C5707_RS27945	118574..118876	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
C5707_RS27950	118881..119231	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5707_RS27955	119394..119942	+	549	WP_001061573.1	hypothetical protein	-
C5707_RS27960	120283..120477	+	195	WP_000343597.1	hypothetical protein	-
C5707_RS27965	120488..120859	+	372	WP_000516918.1	hypothetical protein	-
C5707_RS27970	120852..121322	+	471	WP_001281821.1	hypothetical protein	-
C5707_RS27975	121337..121672	-	336	WP_000683477.1	hypothetical protein	-
C5707_RS27980	121769..122257	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
C5707_RS27985	122260..122757	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / aac(6')-Ib / qacE / sul1 / rmtC / aph(3')-VI / blaNDM-1 / blaCMY-6 / mph(A) / qnrB1 / dfrA14	htpB	1..329160	329160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T156709 WP_000270043.1 NZ_CP053365:c119231-118881 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T156709 NZ_CP069069:c3122449-3122093 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT156709 WP_000124640.1 NZ_CP053365:c118876-118574 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT156709 NZ_CP069069:c3122663-3122433 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9