Detailed information of TA system TA156647

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF3/-
• Location: 2776346..2776653
• Replicon: plasmid unnamed
• Accession: NZ_CP053353
• Organism: Staphylococcus aureus strain 16405

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: HA035_RS13910
• Protein ID: WP_011447039.1
• Coordinates: 2776477..2776653 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	2776346..2776485 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA035_RS13870	2771912..2772091	+	180	WP_000669791.1	hypothetical protein	-
HA035_RS13875	2772402..2772662	+	261	WP_001791826.1	hypothetical protein	-
HA035_RS13880	2772715..2773065	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
HA035_RS13885	2773576..2773911	-	336	Protein_2698	SH3 domain-containing protein	-
HA035_RS13890	2774562..2775053	-	492	WP_000920038.1	staphylokinase	-
HA035_RS13895	2775244..2775999	-	756	WP_000861038.1	CHAP domain-containing protein	-
HA035_RS13900	2776011..2776265	-	255	WP_000611512.1	phage holin	-
HA035_RS13905	2776317..2776424	+	108	WP_031762631.1	hypothetical protein	-
-	2776346..2776485	+	140	NuclAT_0	-	Antitoxin
-	2776346..2776485	+	140	NuclAT_0	-	Antitoxin
-	2776346..2776485	+	140	NuclAT_0	-	Antitoxin
-	2776346..2776485	+	140	NuclAT_0	-	Antitoxin
HA035_RS13910	2776477..2776653	-	177	WP_011447039.1	putative holin-like toxin	Toxin
HA035_RS13915	2776762..2777535	-	774	WP_000750412.1	staphylococcal enterotoxin type A	-
HA035_RS13920	2777908..2778282	-	375	WP_000340977.1	hypothetical protein	-
HA035_RS13925	2778338..2778625	-	288	WP_001262621.1	hypothetical protein	-
HA035_RS13930	2778671..2778823	-	153	WP_001000058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mecA / blaZ / dfrG	hysA / sbi / hlgA / hlgC / hlgB / bpsC / fnbB / fnbA / aur / icaR / icaA / icaD / icaB / icaC / lip / adsA / adsA / spa / cap8A / cap8B / cap8C / cap8D / cap8D / cap8E / cap8E / cap8F / cap8G / cap8L / cap8M / cap8N / cap8O / cap8P / coa / esxA / esaA / essA / esaB / essB / essC / esxC / esxB / esaE / esxD / esaD / esaG4 / esaG1 / esaG7 / esaG3 / esaG3 / esaG4 / esaG9 / geh / clpC / tufA / sdrC / sdrD / sdrE / clpP / vWbp / sspC / sspB / sspA / isdB / isdA / isdC / isdD / isdE / isdF / srtB / isdG / hly/hla / acpXL / katA / ebp / gnd / lukD / hlgA / map / hlb / scn / sak / sea / hlb / groEL / hld	1..2868068	2868068

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T156647 WP_011447039.1 NZ_CP053353:c2776653-2776477 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T156647 NZ_CP069068:c2980215-2979892 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCAGTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin

Nucleotide

• Length: 140 bp

>AT156647 NZ_CP053353:2776346-2776485 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin