Detailed information of TA system TA156602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4915260..4915862
• Replicon: chromosome
• Accession: NZ_CP053296
• Organism: Escherichia coli strain M1/5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HLK60_RS23770
• Protein ID: WP_000897302.1
• Coordinates: 4915551..4915862 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HLK60_RS23765
• Protein ID: WP_000356397.1
• Coordinates: 4915260..4915550 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLK60_RS23740	4911333..4912235	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HLK60_RS23745	4912232..4912867	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HLK60_RS23750	4912864..4913793	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HLK60_RS23755	4914009..4914227	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HLK60_RS23760	4914623..4914901	-	279	WP_001296612.1	hypothetical protein	-
HLK60_RS23765	4915260..4915550	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HLK60_RS23770	4915551..4915862	-	312	WP_000897302.1	hypothetical protein	Toxin
HLK60_RS23775	4916091..4916999	+	909	WP_001331553.1	alpha/beta hydrolase	-
HLK60_RS23780	4917063..4918004	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HLK60_RS23785	4918049..4918486	-	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HLK60_RS23790	4918483..4919355	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HLK60_RS23795	4919349..4919948	-	600	WP_001314324.1	glucose-1-phosphatase	-
HLK60_RS23800	4920047..4920832	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T156602 WP_000897302.1 NZ_CP053296:c4915862-4915551 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T156602 NZ_CP069068:365669-365971 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT156602 WP_000356397.1 NZ_CP053296:c4915550-4915260 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT156602 NZ_CP069068:365445-365672 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: S1P416