Detailed information of TA system TA156596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3904039..3904733
• Replicon: chromosome
• Accession: NZ_CP053296
• Organism: Escherichia coli strain M1/5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HLK60_RS18905
• Protein ID: WP_001263500.1
• Coordinates: 3904039..3904437 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HLK60_RS18910
• Protein ID: WP_000554758.1
• Coordinates: 3904440..3904733 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLK60_RS18880	3899404..3899862	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
HLK60_RS18885	3900123..3901580	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HLK60_RS18890	3901637..3902251	-	615	WP_000602124.1	peptide chain release factor H	-
HLK60_RS18895	3902248..3903387	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
HLK60_RS18900	3903577..3904029	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HLK60_RS18905	3904039..3904437	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HLK60_RS18910	3904440..3904733	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HLK60_RS18915	3904785..3905840	-	1056	Protein_3707	DNA polymerase IV	-
HLK60_RS18920	3905911..3906834	-	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
HLK60_RS18925	3906837..3907700	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
HLK60_RS18930	3907713..3908429	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
HLK60_RS18935	3908449..3908916	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T156596 WP_001263500.1 NZ_CP053296:c3904437-3904039 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156596 NZ_CP069067:3569309-3569653 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT156596 WP_000554758.1 NZ_CP053296:c3904733-3904440 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156596 NZ_CP069067:3569650-3569979 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3