Detailed information of TA system TA156548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4115743..4116437
• Replicon: chromosome
• Accession: NZ_CP053284
• Organism: Escherichia coli strain SCU-104

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: HHJ22_RS20095
• Protein ID: WP_001263489.1
• Coordinates: 4115743..4116141 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ22_RS20100
• Protein ID: WP_000554758.1
• Coordinates: 4116144..4116437 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4111331..4111411	-	81	NuclAT_12	-	-
-	4111331..4111411	-	81	NuclAT_12	-	-
-	4111331..4111411	-	81	NuclAT_12	-	-
-	4111331..4111411	-	81	NuclAT_12	-	-
HHJ22_RS20070	4112007..4112465	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HHJ22_RS20075	4112726..4114183	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
HHJ22_RS20080	4114240..4114797	-	558	WP_001352051.1	peptide chain release factor H	-
HHJ22_RS20085	4114709..4114975	-	267	WP_001295202.1	hypothetical protein	-
HHJ22_RS20090	4115281..4115733	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
HHJ22_RS20095	4115743..4116141	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ22_RS20100	4116144..4116437	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ22_RS20105	4116489..4117544	-	1056	WP_001226164.1	DNA polymerase IV	-
HHJ22_RS20110	4117615..4118400	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HHJ22_RS20115	4118345..4120084	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
HHJ22_RS20120	4120308..4120805	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA	4063643..4131023	67380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T156548 WP_001263489.1 NZ_CP053284:c4116141-4115743 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156548 NZ_CP069067:681379-681786 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT156548 WP_000554758.1 NZ_CP053284:c4116437-4116144 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156548 NZ_CP069067:681167-681382 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3