Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3149620..3150122 Replicon chromosome
Accession NZ_CP053284
Organism Escherichia coli strain SCU-104

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HHJ22_RS15405 Protein ID WP_000767829.1
Coordinates 3149620..3149874 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HHJ22_RS15410 Protein ID WP_001259255.1
Coordinates 3149871..3150122 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ22_RS15375 3144635..3144862 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HHJ22_RS15380 3144876..3145934 - 1059 WP_174147738.1 transport protein YeeE -
HHJ22_RS15385 3146113..3147471 - 1359 WP_024198437.1 putrescine/proton symporter PlaP -
HHJ22_RS15390 3147461..3147523 - 63 WP_010723108.1 membrane protein YoeI -
HHJ22_RS15395 3147738..3148667 - 930 WP_000803351.1 LysR family transcriptional regulator -
HHJ22_RS15400 3148713..3149537 - 825 WP_000754737.1 SDR family oxidoreductase -
HHJ22_RS15405 3149620..3149874 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HHJ22_RS15410 3149871..3150122 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HHJ22_RS15415 3150405..3150455 + 51 WP_001364200.1 his operon leader peptide -
HHJ22_RS15420 3150601..3151500 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HHJ22_RS15425 3151506..3152810 + 1305 WP_174147740.1 histidinol dehydrogenase -
HHJ22_RS15430 3152807..3153877 + 1071 WP_072646327.1 histidinol-phosphate transaminase -
HHJ22_RS15435 3153877..3154944 + 1068 WP_021547024.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T156547 WP_000767829.1 NZ_CP053284:c3149874-3149620 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T156547 NZ_CP069067:c644212-643799 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT156547 WP_001259255.1 NZ_CP053284:c3150122-3149871 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT156547 NZ_CP069067:c644475-644209 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References