Detailed information of TA system TA156515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4775258..4775860
• Replicon: chromosome
• Accession: NZ_CP053281
• Organism: Escherichia coli strain SCU-308

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HHJ45_RS22920
• Protein ID: WP_000897305.1
• Coordinates: 4775549..4775860 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ45_RS22915
• Protein ID: WP_000356397.1
• Coordinates: 4775258..4775548 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ45_RS22890	4771185..4772087	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ45_RS22895	4772084..4772719	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ45_RS22900	4772716..4773645	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HHJ45_RS22905	4773975..4774217	-	243	WP_001087409.1	hypothetical protein	-
HHJ45_RS22910	4774435..4774653	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
HHJ45_RS22915	4775258..4775548	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ45_RS22920	4775549..4775860	-	312	WP_000897305.1	hypothetical protein	Toxin
HHJ45_RS22925	4776089..4776997	+	909	WP_001162704.1	alpha/beta hydrolase	-
HHJ45_RS22930	4777061..4778002	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ45_RS22935	4778047..4778484	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ45_RS22940	4778481..4779353	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ45_RS22945	4779347..4779946	-	600	WP_001295269.1	glucose-1-phosphatase	-
HHJ45_RS22950	4780045..4780830	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T156515 WP_000897305.1 NZ_CP053281:c4775860-4775549 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T156515 NZ_CP069066:2207039-2207416 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT156515 WP_000356397.1 NZ_CP053281:c4775548-4775258 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT156515 NZ_CP069066:2207458-2207907 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7