Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3877353..3877894 Replicon chromosome
Accession NZ_CP053281
Organism Escherichia coli strain SCU-308

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HHJ45_RS18755 Protein ID WP_000615976.1
Coordinates 3877616..3877894 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HHJ45_RS18750 Protein ID WP_000729704.1
Coordinates 3877353..3877613 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ45_RS18725 3873136..3873921 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
HHJ45_RS18730 3873866..3875605 + 1740 WP_001296890.1 flagellar biosynthesis protein FlhA -
HHJ45_RS18735 3875710..3875988 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HHJ45_RS18740 3875981..3876337 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
HHJ45_RS18745 3876394..3877167 - 774 WP_000543895.1 C40 family peptidase -
HHJ45_RS18750 3877353..3877613 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HHJ45_RS18755 3877616..3877894 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HHJ45_RS18760 3878050..3878790 + 741 WP_001225679.1 murein L,D-transpeptidase -
HHJ45_RS18765 3878761..3879528 - 768 WP_000333380.1 class II glutamine amidotransferase -
HHJ45_RS18770 3879734..3880312 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR / rhs/PAAR 3875981..3893904 17923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T156512 WP_000615976.1 NZ_CP053281:3877616-3877894 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T156512 NZ_CP069066:c1944092-1943703 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT156512 WP_000729704.1 NZ_CP053281:3877353-3877613 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT156512 NZ_CP069066:c1944316-1944089 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References