Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1947264..1947805 Replicon chromosome
Accession NZ_CP053258
Organism Escherichia coli strain GF4-3

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag HLY11_RS09265 Protein ID WP_000615979.1
Coordinates 1947527..1947805 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag HLY11_RS09260 Protein ID WP_000729709.1
Coordinates 1947264..1947524 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLY11_RS09240 1943344..1944129 - 786 WP_171459784.1 putative lateral flagellar export/assembly protein LafU -
HLY11_RS09245 1944074..1945813 + 1740 WP_171459785.1 flagellar biosynthesis protein FlhA -
HLY11_RS09250 1945892..1946248 + 357 WP_171460671.1 type II toxin-antitoxin system HicB family antitoxin -
HLY11_RS09255 1946305..1947054 - 750 WP_014639831.1 C40 family peptidase -
HLY11_RS09260 1947264..1947524 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HLY11_RS09265 1947527..1947805 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HLY11_RS09270 1947961..1948701 + 741 WP_001225684.1 murein L,D-transpeptidase -
HLY11_RS09275 1948672..1949439 - 768 WP_000333380.1 class II glutamine amidotransferase -
HLY11_RS09280 1949645..1950223 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T156460 WP_000615979.1 NZ_CP053258:1947527-1947805 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T156460 NZ_CP069065:c2200784-2200488 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT156460 WP_000729709.1 NZ_CP053258:1947264-1947524 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT156460 NZ_CP069065:c2201032-2200781 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References