Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4930216..4930943 Replicon chromosome
Accession NZ_CP053251
Organism Escherichia coli strain SCU-204

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HHJ42_RS23825 Protein ID WP_000550189.1
Coordinates 4930629..4930943 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HHJ42_RS23820 Protein ID WP_000560266.1
Coordinates 4930216..4930632 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ42_RS23810 4925376..4927727 + 2352 WP_000695487.1 alpha-glucosidase -
HHJ42_RS23815 4928153..4930171 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HHJ42_RS23820 4930216..4930632 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HHJ42_RS23825 4930629..4930943 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HHJ42_RS23830 4931226..4932363 - 1138 Protein_4652 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HHJ42_RS23835 4932448..4932951 + 504 WP_001333820.1 M48 family metallopeptidase -
HHJ42_RS23840 4933028..4933720 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HHJ42_RS23845 4933799..4934785 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T156441 WP_000550189.1 NZ_CP053251:c4930943-4930629 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T156441 NZ_CP069065:715716-716123 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT156441 WP_000560266.1 NZ_CP053251:c4930632-4930216 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT156441 NZ_CP069065:715459-715719 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References