Detailed information of TA system TA156440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4865675..4866368
• Replicon: chromosome
• Accession: NZ_CP053251
• Organism: Escherichia coli strain SCU-204

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HHJ42_RS23520
• Protein ID: WP_000415584.1
• Coordinates: 4866072..4866368 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HHJ42_RS23515
• Protein ID: WP_000650107.1
• Coordinates: 4865675..4866070 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ42_RS23505	4861539..4863797	-	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-
HHJ42_RS23510	4863935..4865542	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
HHJ42_RS23515	4865675..4866070	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HHJ42_RS23520	4866072..4866368	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HHJ42_RS23525	4866573..4867055	-	483	WP_000183505.1	transcriptional regulator	-
HHJ42_RS23530	4867108..4867500	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HHJ42_RS23535	4867652..4868311	+	660	WP_001221493.1	two-component system response regulator QseB	-
HHJ42_RS23540	4868308..4869657	+	1350	WP_171274515.1	two-component system sensor histidine kinase QseC	-
HHJ42_RS23545	4869703..4870035	-	333	WP_000917685.1	DUF2645 family protein	-
HHJ42_RS23550	4870354..4870935	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
HHJ42_RS23555	4870966..4871280	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4857212..4866368	9156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T156440 WP_000415584.1 NZ_CP053251:c4866368-4866072 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T156440 NZ_CP069065:714098-714493 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT156440 WP_000650107.1 NZ_CP053251:c4866070-4865675 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT156440 NZ_CP069065:713844-714098 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65