Detailed information of TA system TA156425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1726643..1727337
• Replicon: chromosome
• Accession: NZ_CP053251
• Organism: Escherichia coli strain SCU-204

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HHJ42_RS08225
• Protein ID: WP_001263500.1
• Coordinates: 1726939..1727337 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ42_RS08220
• Protein ID: WP_000554758.1
• Coordinates: 1726643..1726936 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ42_RS08195	1722460..1722927	+	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-
HHJ42_RS08200	1722947..1723663	+	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
HHJ42_RS08205	1723676..1724539	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
HHJ42_RS08210	1724542..1725465	+	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
HHJ42_RS08215	1725536..1726591	+	1056	WP_001226168.1	DNA polymerase IV	-
HHJ42_RS08220	1726643..1726936	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ42_RS08225	1726939..1727337	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ42_RS08230	1727347..1727799	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HHJ42_RS08235	1727977..1729128	+	1152	WP_001335538.1	RNA ligase RtcB family protein	-
HHJ42_RS08240	1729125..1729739	+	615	WP_000602124.1	peptide chain release factor H	-
HHJ42_RS08245	1729796..1731253	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HHJ42_RS08250	1731514..1731972	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T156425 WP_001263500.1 NZ_CP053251:1726939-1727337 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156425 NZ_CP069064:3536640-3536984 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT156425 WP_000554758.1 NZ_CP053251:1726643-1726936 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156425 NZ_CP069064:3536981-3537310 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3