Detailed information of TA system TA156418

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 1335438..1335850
• Replicon: chromosome
• Accession: NZ_CP053251
• Organism: Escherichia coli strain SCU-204

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: HHJ42_RS06365
• Protein ID: WP_000132614.1
• Coordinates: 1335438..1335779 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	1335774..1335850 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ42_RS06345	1330507..1331787	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
HHJ42_RS06350	1331972..1332892	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
HHJ42_RS06355	1333120..1333200	+	81	WP_020233658.1	hypothetical protein	-
HHJ42_RS06360	1333235..1335391	+	2157	WP_001513533.1	DUF262 domain-containing protein	-
HHJ42_RS06365	1335438..1335779	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
-	1335774..1335850	+	77	NuclAT_8	-	Antitoxin
-	1335774..1335850	+	77	NuclAT_8	-	Antitoxin
-	1335774..1335850	+	77	NuclAT_8	-	Antitoxin
-	1335774..1335850	+	77	NuclAT_8	-	Antitoxin
-	1335774..1335850	+	77	NuclAT_9	-	Antitoxin
-	1335774..1335850	+	77	NuclAT_9	-	Antitoxin
-	1335774..1335850	+	77	NuclAT_9	-	Antitoxin
-	1335774..1335850	+	77	NuclAT_9	-	Antitoxin
HHJ42_RS06370	1336000..1337769	-	1770	WP_171274428.1	restriction endonuclease subunit S	-
HHJ42_RS06375	1337769..1339238	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T156418 WP_000132614.1 NZ_CP053251:c1335779-1335438 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T156418 NZ_CP069064:c3051615-3051199 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT156418 NZ_CP053251:1335774-1335850 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin