Detailed information of TA system TA156389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1130766..1131416
• Replicon: chromosome
• Accession: NZ_CP053247
• Organism: Escherichia coli strain SCU-482

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HHJ51_RS05745
• Protein ID: WP_000263532.1
• Coordinates: 1130766..1131107 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: HHJ51_RS05750
• Protein ID: WP_000212552.1
• Coordinates: 1131117..1131416 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ51_RS05720	1125801..1126901	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
HHJ51_RS05725	1126901..1127200	+	300	WP_000867281.1	rod-binding protein	-
HHJ51_RS05730	1127376..1128752	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
HHJ51_RS05735	1128767..1129696	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
HHJ51_RS05740	1129713..1130690	+	978	WP_001195938.1	hypothetical protein	-
HHJ51_RS05745	1130766..1131107	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ51_RS05750	1131117..1131416	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
HHJ51_RS05755	1131474..1132316	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
HHJ51_RS05760	1132802..1133716	+	915	WP_000949083.1	lateral flagellin LafA	-
HHJ51_RS05765	1133921..1135237	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
HHJ51_RS05770	1135260..1135652	+	393	WP_000285322.1	flagellar export chaperone FliS	-
HHJ51_RS05775	1135657..1135968	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T156389 WP_000263532.1 NZ_CP053247:1130766-1131107 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T156389 NZ_CP069064:c752994-752557 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT156389 WP_000212552.1 NZ_CP053247:1131117-1131416 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT156389 NZ_CP069064:c753245-752991 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7