Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4163227..4163789 Replicon chromosome
Accession NZ_CP053245
Organism Escherichia coli strain SCU-485

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HHJ54_RS19830 Protein ID WP_000605675.1
Coordinates 4163511..4163789 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ54_RS19825 Protein ID WP_000781370.1
Coordinates 4163227..4163511 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ54_RS19810 4158408..4161455 + 3048 WP_077627348.1 formate dehydrogenase-N subunit alpha -
HHJ54_RS19815 4161468..4162352 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HHJ54_RS19820 4162345..4162998 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HHJ54_RS19825 4163227..4163511 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HHJ54_RS19830 4163511..4163789 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ54_RS19835 4163975..4164985 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HHJ54_RS19840 4165119..4166816 - 1698 WP_000433462.1 malate dehydrogenase -
HHJ54_RS19845 4166972..4167109 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ54_RS19850 4167211..4167426 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ54_RS19855 4167773..4168204 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T156375 WP_000605675.1 NZ_CP053245:c4163789-4163511 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T156375 NZ_CP069064:359673-359975 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT156375 WP_000781370.1 NZ_CP053245:c4163511-4163227 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT156375 NZ_CP069064:359449-359676 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References