Detailed information of TA system TA156368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2900416..2901110
• Replicon: chromosome
• Accession: NZ_CP053245
• Organism: Escherichia coli strain SCU-485

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HHJ54_RS13505
• Protein ID: WP_001263500.1
• Coordinates: 2900712..2901110 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ54_RS13500
• Protein ID: WP_000554758.1
• Coordinates: 2900416..2900709 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ54_RS13480	2896152..2896649	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
HHJ54_RS13485	2896769..2898508	-	1740	WP_001337446.1	flagellar biosynthesis protein FlhA	-
HHJ54_RS13490	2898453..2899238	+	786	WP_000207543.1	putative lateral flagellar export/assembly protein LafU	-
HHJ54_RS13495	2899309..2900364	+	1056	WP_001226162.1	DNA polymerase IV	-
HHJ54_RS13500	2900416..2900709	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ54_RS13505	2900712..2901110	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ54_RS13510	2901120..2901572	+	453	WP_001059897.1	GNAT family N-acetyltransferase	-
HHJ54_RS13515	2901750..2902901	+	1152	WP_023145556.1	RNA ligase RtcB family protein	-
HHJ54_RS13520	2902898..2903512	+	615	WP_000602123.1	peptide chain release factor H	-
HHJ54_RS13525	2903569..2905026	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HHJ54_RS13530	2905287..2905745	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2870166..2901572	31406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T156368 WP_001263500.1 NZ_CP053245:2900712-2901110 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156368 NZ_CP069063:3177732-3178175 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT156368 WP_000554758.1 NZ_CP053245:2900416-2900709 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156368 NZ_CP069063:3177488-3177745 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3