Detailed information of TA system TA156362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2028432..2029034
• Replicon: chromosome
• Accession: NZ_CP053245
• Organism: Escherichia coli strain SCU-485

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ54_RS09505
• Protein ID: WP_000897302.1
• Coordinates: 2028432..2028743 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ54_RS09510
• Protein ID: WP_000356397.1
• Coordinates: 2028744..2029034 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ54_RS09475	2023462..2024247	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
HHJ54_RS09480	2024346..2024945	+	600	WP_001314324.1	glucose-1-phosphatase	-
HHJ54_RS09485	2024939..2025811	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ54_RS09490	2025808..2026245	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ54_RS09495	2026290..2027231	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ54_RS09500	2027295..2028203	-	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ54_RS09505	2028432..2028743	+	312	WP_000897302.1	hypothetical protein	Toxin
HHJ54_RS09510	2028744..2029034	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ54_RS09515	2029393..2029671	+	279	WP_001296612.1	hypothetical protein	-
HHJ54_RS09520	2030067..2030285	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ54_RS09525	2030501..2031430	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ54_RS09530	2031427..2032062	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ54_RS09535	2032059..2032961	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T156362 WP_000897302.1 NZ_CP053245:2028432-2028743 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T156362 NZ_CP069063:c3065508-3065092 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT156362 WP_000356397.1 NZ_CP053245:2028744-2029034 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT156362 NZ_CP069063:c3065771-3065505 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: S1P416