Detailed information of TA system TA156351

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76856..77281
• Replicon: plasmid pSCU-106-2
• Accession: NZ_CP053236
• Organism: Escherichia coli strain SCU-106

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: HHJ24_RS26510
• Protein ID: WP_001332444.1
• Coordinates: 76856..77017 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	77070..77281 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ24_RS26480	72461..72937	-	477	WP_170969613.1	PAS domain-containing protein	-
HHJ24_RS26485	73127..73510	-	384	WP_000124981.1	relaxosome protein TraM	-
HHJ24_RS26490	73925..74434	+	510	WP_000759163.1	transglycosylase SLT domain-containing protein	-
HHJ24_RS26495	74730..75551	-	822	WP_001234468.1	DUF945 domain-containing protein	-
HHJ24_RS26500	75670..75957	-	288	WP_000107520.1	hypothetical protein	-
HHJ24_RS26505	76021..76257	+	237	WP_001337416.1	hypothetical protein	-
HHJ24_RS26510	76856..77017	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	77070..77281	-	212	NuclAT_0	-	Antitoxin
-	77070..77281	-	212	NuclAT_0	-	Antitoxin
-	77070..77281	-	212	NuclAT_0	-	Antitoxin
-	77070..77281	-	212	NuclAT_0	-	Antitoxin
HHJ24_RS26515	77293..78012	-	720	WP_025670723.1	plasmid SOS inhibition protein A	-
HHJ24_RS26520	78009..78443	-	435	WP_000845922.1	conjugation system SOS inhibitor PsiB	-
HHJ24_RS26525	78498..80456	-	1959	WP_170969614.1	ParB/RepB/Spo0J family partition protein	-
HHJ24_RS26530	80520..80753	-	234	WP_001553739.1	DUF905 family protein	-
HHJ24_RS26535	80811..81350	-	540	WP_025670725.1	single-stranded DNA-binding protein	-
HHJ24_RS26540	81376..81582	-	207	WP_000547969.1	hypothetical protein	-
HHJ24_RS26545	81652..82077	+	426	WP_071791129.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA	1..111501	111501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T156351 WP_001332444.1 NZ_CP053236:c77017-76856 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T156351 NZ_CP069063:c2242268-2241924 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Nucleotide

• Length: 212 bp

>AT156351 NZ_CP053236:c77281-77070 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin