Detailed information of TA system TA156348

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51238..51492
• Replicon: plasmid pSCU-106-2
• Accession: NZ_CP053236
• Organism: Escherichia coli strain SCU-106

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HHJ24_RS26385
• Protein ID: WP_001351576.1
• Coordinates: 51238..51444 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	51431..51492 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ24_RS26360	48098..48721	-	624	WP_000904945.1	recombinase family protein	-
HHJ24_RS26365	49066..49923	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HHJ24_RS26370	49916..50398	-	483	WP_001273588.1	hypothetical protein	-
HHJ24_RS26375	50391..50465	-	75	WP_001442103.1	RepA leader peptide Tap	-
HHJ24_RS26380	50697..50954	-	258	WP_000083845.1	replication regulatory protein RepA	-
HHJ24_RS26385	51238..51444	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	51431..51492	+	62	NuclAT_1	-	Antitoxin
-	51431..51492	+	62	NuclAT_1	-	Antitoxin
-	51431..51492	+	62	NuclAT_1	-	Antitoxin
-	51431..51492	+	62	NuclAT_1	-	Antitoxin
HHJ24_RS26390	51748..51822	-	75	Protein_57	endonuclease	-
HHJ24_RS26395	52068..52280	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HHJ24_RS26400	52416..52976	-	561	WP_025670714.1	fertility inhibition protein FinO	-
HHJ24_RS26405	53079..53939	-	861	WP_000704513.1	alpha/beta hydrolase	-
HHJ24_RS26410	53998..54744	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA	1..111501	111501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T156348 WP_001351576.1 NZ_CP053236:c51444-51238 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T156348 NZ_CP069063:c2207107-2206811 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGCGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT156348 NZ_CP053236:51431-51492 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin