Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4620976..4621478 Replicon chromosome
Accession NZ_CP053234
Organism Escherichia coli strain SCU-106

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HHJ24_RS22300 Protein ID WP_000767829.1
Coordinates 4621224..4621478 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HHJ24_RS22295 Protein ID WP_001259255.1
Coordinates 4620976..4621227 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ24_RS22270 4616153..4617220 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HHJ24_RS22275 4617220..4618290 - 1071 WP_106918456.1 histidinol-phosphate transaminase -
HHJ24_RS22280 4618287..4619591 - 1305 WP_021562278.1 histidinol dehydrogenase -
HHJ24_RS22285 4619597..4620496 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HHJ24_RS22290 4620642..4620692 - 51 WP_001364200.1 his operon leader peptide -
HHJ24_RS22295 4620976..4621227 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HHJ24_RS22300 4621224..4621478 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HHJ24_RS22305 4621561..4622385 + 825 WP_021562277.1 SDR family oxidoreductase -
HHJ24_RS22310 4622431..4623360 + 930 WP_000803351.1 LysR family transcriptional regulator -
HHJ24_RS22315 4623575..4623637 + 63 WP_010723108.1 membrane protein YoeI -
HHJ24_RS22320 4623627..4624985 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HHJ24_RS22325 4625164..4626222 + 1059 WP_000492346.1 transport protein YeeE -
HHJ24_RS22330 4626236..4626463 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T156345 WP_000767829.1 NZ_CP053234:4621224-4621478 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T156345 NZ_CP069063:1964930-1965178 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT156345 WP_001259255.1 NZ_CP053234:4620976-4621227 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT156345 NZ_CP069063:1964718-1964930 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References