Detailed information of TA system TA156338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2517250..2517852
• Replicon: chromosome
• Accession: NZ_CP053234
• Organism: Escherichia coli strain SCU-106

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HHJ24_RS12255
• Protein ID: WP_000897305.1
• Coordinates: 2517541..2517852 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ24_RS12250
• Protein ID: WP_000356397.1
• Coordinates: 2517250..2517540 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ24_RS12225	2513176..2514078	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ24_RS12230	2514075..2514710	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ24_RS12235	2514707..2515636	+	930	WP_170969412.1	formate dehydrogenase accessory protein FdhE	-
HHJ24_RS12240	2515966..2516208	-	243	WP_001086388.1	hypothetical protein	-
HHJ24_RS12245	2516427..2516645	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
HHJ24_RS12250	2517250..2517540	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ24_RS12255	2517541..2517852	-	312	WP_000897305.1	hypothetical protein	Toxin
HHJ24_RS12260	2518081..2518989	+	909	WP_001162704.1	alpha/beta hydrolase	-
HHJ24_RS12265	2519053..2519994	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ24_RS12270	2520039..2520476	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ24_RS12275	2520473..2521345	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ24_RS12280	2521339..2521938	-	600	WP_001315111.1	glucose-1-phosphatase	-
HHJ24_RS12285	2522037..2522822	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T156338 WP_000897305.1 NZ_CP053234:c2517852-2517541 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T156338 NZ_CP069063:1384981-1385412 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT156338 WP_000356397.1 NZ_CP053234:c2517540-2517250 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT156338 NZ_CP069063:1384724-1384984 [Mycobacterium tuberculosis]
ATGCGCACCACCTGCACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7