Detailed information of TA system TA156320

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 118925..119351
• Replicon: plasmid pSCU-306-1
• Accession: NZ_CP053232
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHJ44_RS24925
• Protein ID: WP_001312861.1
• Coordinates: 118925..119083 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	119127..119351 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS24895	114316..115010	-	695	Protein_134	IS1 family transposase	-
HHJ44_RS24900	115181..115564	-	384	WP_000124983.1	relaxosome protein TraM	-
HHJ44_RS24905	115841..116488	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
HHJ44_RS24910	116785..117606	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HHJ44_RS24915	117727..118014	-	288	WP_000107537.1	hypothetical protein	-
HHJ44_RS24920	118078..118306	+	229	Protein_139	hypothetical protein	-
HHJ44_RS24925	118925..119083	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	119127..119351	-	225	NuclAT_0	-	Antitoxin
-	119127..119351	-	225	NuclAT_0	-	Antitoxin
-	119127..119351	-	225	NuclAT_0	-	Antitoxin
-	119127..119351	-	225	NuclAT_0	-	Antitoxin
HHJ44_RS24930	119163..119351	+	189	WP_001299721.1	hypothetical protein	-
HHJ44_RS24935	119363..120082	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
HHJ44_RS24940	120079..120513	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
HHJ44_RS24945	120568..122526	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
HHJ44_RS24950	122585..122818	-	234	WP_000006004.1	DUF905 domain-containing protein	-
HHJ44_RS24955	122874..123401	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / aadA2 / dfrA12	senB	1..128584	128584
	-	flank	IS/Tn	-	-	114359..114691	332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T156320 WP_001312861.1 NZ_CP053232:c119083-118925 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T156320 NZ_CP069063:c447610-447017 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT156320 NZ_CP053232:c119351-119127 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin