Detailed information of TA system TA156316

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105755..106009
• Replicon: plasmid pSCU-306-1
• Accession: NZ_CP053232
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HHJ44_RS24815
• Protein ID: WP_001362723.1
• Coordinates: 105803..106009 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	105755..105816 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS24790	102930..103676	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
HHJ44_RS24795	103731..104291	+	561	WP_000139325.1	fertility inhibition protein FinO	-
HHJ44_RS24800	104423..104635	+	213	WP_001301409.1	ANR family transcriptional regulator	-
HHJ44_RS24805	104881..104967	+	87	Protein_116	endonuclease	-
HHJ44_RS24810	105148..105459	-	312	WP_000802277.1	hypothetical protein	-
-	105755..105816	-	62	NuclAT_1	-	Antitoxin
-	105755..105816	-	62	NuclAT_1	-	Antitoxin
-	105755..105816	-	62	NuclAT_1	-	Antitoxin
-	105755..105816	-	62	NuclAT_1	-	Antitoxin
HHJ44_RS24815	105803..106009	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ44_RS24820	106293..106550	+	258	WP_000083845.1	replication regulatory protein RepA	-
HHJ44_RS24825	106785..106859	+	75	WP_057113837.1	RepA leader peptide Tap	-
HHJ44_RS24830	106852..107448	+	597	Protein_121	incFII family plasmid replication initiator RepA	-
HHJ44_RS24835	107500..108204	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HHJ44_RS24840	108269..108640	-	372	Protein_123	type IV conjugative transfer system lipoprotein TraV	-
HHJ44_RS24845	108637..108888	-	252	WP_001038341.1	conjugal transfer protein TrbG	-
HHJ44_RS24850	108885..109202	-	318	WP_001057292.1	conjugal transfer protein TrbD	-
HHJ44_RS24855	109199..109771	-	573	WP_024179131.1	conjugal transfer protein TraP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / aadA2 / dfrA12	senB	1..128584	128584
	-	flank	IS/Tn	-	-	107500..108204	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T156316 WP_001362723.1 NZ_CP053232:105803-106009 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T156316 NZ_CP069063:71516-71917 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Nucleotide

• Length: 62 bp

>AT156316 NZ_CP053232:c105816-105755 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin