Detailed information of TA system TA156313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4702488..4703167
• Replicon: chromosome
• Accession: NZ_CP053231
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0VBP6
• Locus tag: HHJ44_RS22640
• Protein ID: WP_000057524.1
• Coordinates: 4702488..4702790 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: HHJ44_RS22645
• Protein ID: WP_000806442.1
• Coordinates: 4702826..4703167 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS22615	4697864..4699516	+	1653	WP_000771762.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HHJ44_RS22620	4699554..4700057	-	504	WP_000667000.1	hypothetical protein	-
HHJ44_RS22625	4700054..4700854	-	801	WP_000439796.1	hypothetical protein	-
HHJ44_RS22630	4700878..4701357	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HHJ44_RS22635	4701561..4702355	-	795	WP_000365177.1	TraB/GumN family protein	-
HHJ44_RS22640	4702488..4702790	+	303	WP_000057524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ44_RS22645	4702826..4703167	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
HHJ44_RS22650	4703225..4705729	-	2505	WP_000083945.1	copper-exporting P-type ATPase CopA	-
HHJ44_RS22655	4705991..4706923	+	933	WP_000883052.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4699554..4711767	12213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11839.47 Da
• Isoelectric Point: 10.2237

>T156313 WP_000057524.1 NZ_CP053231:4702488-4702790 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T156313 NZ_CP069062:3777878-3778135 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT156313 WP_000806442.1 NZ_CP053231:4702826-4703167 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT156313 NZ_CP069062:3777606-3777881 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• AlphaFold DB: V0VBP6

Antitoxin

• AlphaFold DB: S1QAY3