Detailed information of TA system TA156311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4470933..4471627
• Replicon: chromosome
• Accession: NZ_CP053231
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HHJ44_RS21565
• Protein ID: WP_001263500.1
• Coordinates: 4471229..4471627 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ44_RS21560
• Protein ID: WP_000554758.1
• Coordinates: 4470933..4471226 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS21535	4466469..4466966	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
HHJ44_RS21540	4467049..4467207	-	159	WP_014639450.1	hypothetical protein	-
HHJ44_RS21545	4467286..4469025	-	1740	WP_001331867.1	flagellar biosynthesis protein FlhA	-
HHJ44_RS21550	4468970..4469755	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
HHJ44_RS21555	4469826..4470881	+	1056	WP_001226168.1	DNA polymerase IV	-
HHJ44_RS21560	4470933..4471226	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ44_RS21565	4471229..4471627	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ44_RS21570	4471637..4472089	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HHJ44_RS21575	4472267..4473418	+	1152	WP_001331869.1	RNA ligase RtcB family protein	-
HHJ44_RS21580	4473415..4474029	+	615	WP_000602123.1	peptide chain release factor H	-
HHJ44_RS21585	4474086..4475543	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HHJ44_RS21590	4475804..4476262	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	4453497..4472089	18592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T156311 WP_001263500.1 NZ_CP053231:4471229-4471627 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156311 NZ_CP069062:3553927-3554271 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT156311 WP_000554758.1 NZ_CP053231:4470933-4471226 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156311 NZ_CP069062:3554268-3554597 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3