Detailed information of TA system TA156302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3446673..3447275
• Replicon: chromosome
• Accession: NZ_CP053231
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ44_RS16665
• Protein ID: WP_000897302.1
• Coordinates: 3446673..3446984 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ44_RS16670
• Protein ID: WP_000356397.1
• Coordinates: 3446985..3447275 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS16640	3442587..3443186	+	600	WP_001314324.1	glucose-1-phosphatase	-
HHJ44_RS16645	3443180..3444052	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ44_RS16650	3444049..3444486	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ44_RS16655	3444531..3445472	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ44_RS16660	3445536..3446444	-	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ44_RS16665	3446673..3446984	+	312	WP_000897302.1	hypothetical protein	Toxin
HHJ44_RS16670	3446985..3447275	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ44_RS16675	3447634..3447912	+	279	WP_001296612.1	hypothetical protein	-
HHJ44_RS16680	3448308..3448526	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ44_RS16685	3448742..3449671	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ44_RS16690	3449668..3450303	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ44_RS16695	3450300..3451202	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T156302 WP_000897302.1 NZ_CP053231:3446673-3446984 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T156302 NZ_CP069062:c2878662-2878267 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT156302 WP_000356397.1 NZ_CP053231:3446985-3447275 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT156302 NZ_CP069062:c2878904-2878659 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• AlphaFold DB: S1P416