Detailed information of TA system TA156277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 27908..28370
• Replicon: plasmid unnamed1
• Accession: NZ_CP053219
• Organism: Acinetobacter baumannii strain DT01139C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: HLG77_RS18050
• Protein ID: WP_000033474.1
• Coordinates: 27908..28096 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HLG77_RS18055
• Protein ID: WP_001258992.1
• Coordinates: 28083..28370 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLG77_RS18015	24314..24670	+	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HLG77_RS18020	24663..24965	+	303	WP_001140619.1	XRE family transcriptional regulator	-
HLG77_RS18025	24958..25167	+	210	WP_000069471.1	hypothetical protein	-
HLG77_RS18030	25275..25502	-	228	WP_000286812.1	hypothetical protein	-
HLG77_RS18035	26261..26806	-	546	Protein_31	IS5-like element ISAba13 family transposase	-
HLG77_RS18040	26734..27123	-	390	WP_002072923.1	AAA family ATPase	-
HLG77_RS18045	27311..27631	+	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HLG77_RS18050	27908..28096	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HLG77_RS18055	28083..28370	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
HLG77_RS18060	28449..28703	-	255	WP_000236586.1	hypothetical protein	-
HLG77_RS18065	28654..28878	-	225	WP_004882762.1	hypothetical protein	-
HLG77_RS18070	28917..29459	-	543	Protein_38	hypothetical protein	-
HLG77_RS18075	29499..30811	-	1313	Protein_39	terminase	-
HLG77_RS18080	30795..31336	-	542	Protein_40	DUF2280 domain-containing protein	-
HLG77_RS18085	31396..32001	-	606	Protein_41	hypothetical protein	-
HLG77_RS18090	32100..33308	+	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97161	97161
	-	inside	IScluster/Tn	-	-	19081..26686	7605
	-	flank	IS/Tn	-	-	32100..33308	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T156277 WP_000033474.1 NZ_CP053219:27908-28096 [Acinetobacter baumannii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T156277 NZ_CP069062:1170256-1171137 [Mycobacterium tuberculosis]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT156277 WP_001258992.1 NZ_CP053219:28083-28370 [Acinetobacter baumannii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT156277 NZ_CP069062:1169636-1170259 [Mycobacterium tuberculosis]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35