Detailed information of TA system TA156276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24314..24965
• Replicon: plasmid unnamed1
• Accession: NZ_CP053219
• Organism: Acinetobacter baumannii strain DT01139C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0E2G754
• Locus tag: HLG77_RS18015
• Protein ID: WP_000269901.1
• Coordinates: 24314..24670 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HLG77_RS18020
• Protein ID: WP_001140619.1
• Coordinates: 24663..24965 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLG77_RS18000	20564..21082	-	519	WP_000447197.1	sel1 repeat family protein	-
HLG77_RS18005	21761..22273	+	513	WP_179993820.1	SMI1/KNR4 family protein	-
HLG77_RS18010	22437..23762	+	1326	WP_001280604.1	ISNCY family transposase	-
HLG77_RS18015	24314..24670	+	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HLG77_RS18020	24663..24965	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
HLG77_RS18025	24958..25167	+	210	WP_000069471.1	hypothetical protein	-
HLG77_RS18030	25275..25502	-	228	WP_000286812.1	hypothetical protein	-
HLG77_RS18035	26261..26806	-	546	Protein_31	IS5-like element ISAba13 family transposase	-
HLG77_RS18040	26734..27123	-	390	WP_002072923.1	AAA family ATPase	-
HLG77_RS18045	27311..27631	+	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HLG77_RS18050	27908..28096	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HLG77_RS18055	28083..28370	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-
HLG77_RS18060	28449..28703	-	255	WP_000236586.1	hypothetical protein	-
HLG77_RS18065	28654..28878	-	225	WP_004882762.1	hypothetical protein	-
HLG77_RS18070	28917..29459	-	543	Protein_38	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97161	97161
	-	inside	IScluster/Tn	-	-	19081..26686	7605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13487.44 Da
• Isoelectric Point: 6.4631

>T156276 WP_000269901.1 NZ_CP053219:24314-24670 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T156276 NZ_CP069062:1017448-1017882 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT156276 WP_001140619.1 NZ_CP053219:24663-24965 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT156276 NZ_CP069062:1017263-1017442 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• AlphaFold DB: A0A0E2G754