Detailed information of TA system TA156265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3593499..3594193
• Replicon: chromosome
• Accession: NZ_CP053211
• Organism: Escherichia coli strain QDFD216

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HJ589_RS17530
• Protein ID: WP_001263493.1
• Coordinates: 3593499..3593897 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HJ589_RS17535
• Protein ID: WP_000554757.1
• Coordinates: 3593900..3594193 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3589159)	3589159..3589239	-	81	NuclAT_12	-	-
- (3589159)	3589159..3589239	-	81	NuclAT_12	-	-
- (3589159)	3589159..3589239	-	81	NuclAT_12	-	-
- (3589159)	3589159..3589239	-	81	NuclAT_12	-	-
HJ589_RS17500 (3588499)	3588499..3589743	-	1245	WP_000189541.1	esterase FrsA	-
HJ589_RS17505 (3589835)	3589835..3590293	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HJ589_RS17510 (3590554)	3590554..3592011	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HJ589_RS17515 (3592068)	3592068..3592589	-	522	Protein_3472	peptide chain release factor H	-
HJ589_RS17520 (3592588)	3592588..3592791	-	204	Protein_3473	RtcB family protein	-
HJ589_RS17525 (3593037)	3593037..3593489	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HJ589_RS17530 (3593499)	3593499..3593897	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HJ589_RS17535 (3593900)	3593900..3594193	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HJ589_RS17540 (3594245)	3594245..3595300	-	1056	WP_114505516.1	DNA polymerase IV	-
HJ589_RS17545 (3595371)	3595371..3596156	-	786	Protein_3478	putative lateral flagellar export/assembly protein LafU	-
HJ589_RS17550 (3596128)	3596128..3597840	+	1713	Protein_3479	flagellar biosynthesis protein FlhA	-
HJ589_RS17555 (3598064)	3598064..3598561	-	498	WP_114505517.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T156265 WP_001263493.1 NZ_CP053211:c3593897-3593499 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156265 NZ_CP069062:c698100-697708 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT156265 WP_000554757.1 NZ_CP053211:c3594193-3593900 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156265 NZ_CP069062:c698354-698097 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6