Detailed information of TA system TA156238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13600..14257
• Replicon: plasmid pMS-37b
• Accession: NZ_CP053192
• Organism: Enterobacter hormaechei strain EGYMCRVIM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: HK406_RS24005
• Protein ID: WP_000270043.1
• Coordinates: 13907..14257 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HK406_RS24000
• Protein ID: WP_000124640.1
• Coordinates: 13600..13902 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HK406_RS23950	9234..9662	+	429	WP_057729316.1	hypothetical protein	-
HK406_RS23955	9720..10079	+	360	WP_000422769.1	hypothetical protein	-
HK406_RS23960	10079..10525	+	447	WP_000919343.1	hypothetical protein	-
HK406_RS23965	10522..11040	+	519	WP_000210757.1	nitrite reductase	-
HK406_RS23970	11040..11270	+	231	WP_000972665.1	hypothetical protein	-
HK406_RS23975	11257..12114	+	858	WP_038268741.1	hypothetical protein	-
HK406_RS25415	12026..12331	+	306	WP_171820680.1	hypothetical protein	-
HK406_RS23985	12334..12861	+	528	WP_001210659.1	thermonuclease family protein	-
HK406_RS23990	12919..13191	+	273	WP_001043046.1	HU family DNA-binding protein	-
HK406_RS23995	13280..13573	+	294	WP_001239998.1	hypothetical protein	-
HK406_RS24000	13600..13902	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
HK406_RS24005	13907..14257	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HK406_RS24010	14420..14968	+	549	WP_038268731.1	hypothetical protein	-
HK406_RS24020	15309..15503	+	195	WP_038268727.1	hypothetical protein	-
HK406_RS24025	15514..15885	+	372	WP_170851374.1	hypothetical protein	-
HK406_RS24030	15878..16348	+	471	WP_001281821.1	hypothetical protein	-
HK406_RS24035	16363..16698	-	336	WP_000683477.1	hypothetical protein	-
HK406_RS24040	16795..17283	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
HK406_RS24045	17286..17783	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..129016	129016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T156238 WP_000270043.1 NZ_CP053192:c14257-13907 [Enterobacter hormaechei]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T156238 NZ_CP069005:65987-66505 [Sulfitobacter mediterraneus]
ATGAGCGGCGATAGCGTGCCCGCCCAAGCGCCCCTTGTCGTAAACGGATGGTCGATTTATGCGCATCCGCTCTTTCTGGA
CCAGCTCGAAGGGCTGATCCTGGAGGTCGAGGCGCGTAAGGCACGCGATCCAAAGACCTGGCGCAAGAAAAACCCCACGA
AACGGCTGGCAGCCATCTTCAAGCTGGTCACCGAGGCCATACCGGCGGACCCGGGTGCCGCCGCCTACCGGCAAGGCAGC
ACGCTCGGGGATCACCGCAAGCACTGGTTCCGGGCGAAATTCTTCCAGCAGTATCGGCTGTTCTATCGGTTCAACAGCGA
TGCGAAGGTCATCGTGGTGGCCTGGGTGAACGACGACAAGACCCTGCGCGCCTACGGCAGCAAGACGGATGCCTATGCGA
CGTTCAAAGGGATGTTGGAAGATGGCAATCCACCTGACAATTTCGACGCGCTCTTGAAAGAAGCTGCGGCGGCGGACAAG
CGGTTTGAGACGTCTCTTGAAGCGGCGCCCGATCGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT156238 WP_000124640.1 NZ_CP053192:c13902-13600 [Enterobacter hormaechei]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT156238 NZ_CP069005:65664-65990 [Sulfitobacter mediterraneus]
ATGACAGCGCTTGCACAAGATGTCTCGAAACTTACGGATCGGTATCAGACGACCGTGCCGGCGGGTGTGCGCAAGCAGCT
CAAGCTGGGCAAGGGCGACCAGATTCGCTACTGTACCGAGCCGAGTGGCAGGGTCTATATCGAGCCCGTGCGCAGCGACG
AGGAAGATCCCGTGCTCGGCGCCTTTCTCGATTTTGTCGAGGCCGATATCAAGGCGCATCCGGATCGCATTCGGGCGTTC
GATGGGGCTTTGCATGGCCGTCTTGCAGCACTGGTCGGAGACGTTGACGTCGATCTCGATGGGCCGCTATCGCTCGAGGA
TGAATGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9