Detailed information of TA system TA156222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31733..32463
• Replicon: chromosome
• Accession: NZ_CP053190
• Organism: Enterobacter hormaechei strain EGYMCRVIM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: HK406_RS00155
• Protein ID: WP_023302825.1
• Coordinates: 31733..32047 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HK406_RS00160
• Protein ID: WP_127350686.1
• Coordinates: 32044..32463 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HK406_RS00130	27399..28484	+	1086	Protein_25	cellulase family glycosylhydrolase	-
HK406_RS00135	28390..29574	-	1185	WP_048245027.1	multidrug efflux MFS transporter EmrD	-
HK406_RS00140	29750..30583	-	834	WP_023323922.1	DMT family transporter	-
HK406_RS00145	30646..31092	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
HK406_RS00150	31157..31246	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
HK406_RS00155	31733..32047	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
HK406_RS00160	32044..32463	+	420	WP_127350686.1	helix-turn-helix domain-containing protein	Antitoxin
HK406_RS00165	32610..32708	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
HK406_RS00170	32815..34503	+	1689	WP_058658994.1	acetolactate synthase large subunit	-
HK406_RS00175	34507..34794	+	288	WP_003861055.1	acetolactate synthase small subunit	-
HK406_RS00180	34877..35470	+	594	WP_003861054.1	transcriptional regulator UhpA	-
HK406_RS00185	35467..36972	+	1506	WP_003861052.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T156222 WP_023302825.1 NZ_CP053190:31733-32047 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T156222 NZ_CP068983:c2488281-2488027 [Paradevosia shaoguanensis]
ATGCGCACGAAATCCATTTCCGACTTCCGCAAGAACATTGCCGAGGACATCAACGATGTCGTCGAGGACCATGTGCCGCT
CCTCATCACCCGCGGCGGCGGCAAGCCCGCAGCCGTGGTGATGTCGCTTGAGGACTTTGCCTCGTGGCAGGAGACGAACT
ACCTGCTTGGCAGCCCCAAGAATGCCAAGCGTCTCCTGCGCTCGATCAGGCAGCTGGAAGAAGGCAAGGCGACGGAGCGG
AAGCTCATAGAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15502.15 Da
• Isoelectric Point: 6.4840

>AT156222 WP_127350686.1 NZ_CP053190:32044-32463 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLHIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT156222 NZ_CP068983:c2488030-2487776 [Paradevosia shaoguanensis]
GTGAAGCTCACCTTCTCCGATGACGGCTGGGAGGATTATCTGCACTGGCAGGCGCAGGACAAGAAGCTGCTGAAGCGGCT
CAATGCCTTGATCAAGGAATGCCAGCGGACCCCATTTTCAGGCACAGGCAAACCGGAGCCTCTAAAGGGGGAATTGTCCG
GATACTGGTCGCGCCGCCTCGATCAGGAGCATCGGCTCGTTTACCGCTACGCTGAAGACGCGCTCGAAATAGTCCAGTGC
CGTAAACACTACTAG

Structures

Toxin

• AlphaFold DB: A0A837FIL2