Detailed information of TA system TA156159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14617..15274
• Replicon: plasmid pEc61B
• Accession: NZ_CP053105
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain Ec61

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: HLA96_RS24270
• Protein ID: WP_000270043.1
• Coordinates: 14924..15274 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HLA96_RS24265
• Protein ID: WP_000124640.1
• Coordinates: 14617..14919 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLA96_RS24220	9722..10084	+	363	WP_021293829.1	hypothetical protein	-
HLA96_RS24225	10077..11075	+	999	WP_021293828.1	hypothetical protein	-
HLA96_RS24230	11085..11531	+	447	WP_021293827.1	Fe3+-siderophore ABC transporter permease	-
HLA96_RS24235	11528..12046	+	519	WP_021293826.1	nitrite reductase	-
HLA96_RS24240	12046..12276	+	231	WP_197620809.1	hypothetical protein	-
HLA96_RS24245	12263..13120	+	858	WP_021293824.1	hypothetical protein	-
HLA96_RS24250	13351..13878	+	528	WP_011872875.1	thermonuclease family protein	-
HLA96_RS24255	13936..14208	+	273	WP_001043047.1	HU family DNA-binding protein	-
HLA96_RS24260	14297..14590	+	294	WP_001239997.1	chromosome segregation protein ParM	-
HLA96_RS24265	14617..14919	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
HLA96_RS24270	14924..15274	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HLA96_RS24275	15437..15985	+	549	WP_001061574.1	transcriptional regulator	-
HLA96_RS24280	16326..16520	+	195	WP_000343597.1	hypothetical protein	-
HLA96_RS24285	16531..16902	+	372	WP_000516916.1	hypothetical protein	-
HLA96_RS24290	16895..17365	+	471	WP_001281821.1	hypothetical protein	-
HLA96_RS24295	17380..17715	-	336	WP_000683476.1	hypothetical protein	-
HLA96_RS24300	17812..18300	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
HLA96_RS24305	18303..18800	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(D) / blaSHV-5 / sul1 / qacE / ant(3'')-Ia / tet(C) / sul2	-	1..77651	77651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T156159 WP_000270043.1 NZ_CP053105:c15274-14924 [Enterobacter hormaechei subsp. xiangfangensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T156159 NZ_CP068829:2634908-2636230 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
TGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGGAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGGTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGCCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT156159 WP_000124640.1 NZ_CP053105:c14919-14617 [Enterobacter hormaechei subsp. xiangfangensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT156159 NZ_CP068829:2634642-2634908 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9