Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3570714..3571371 Replicon chromosome
Accession NZ_CP053103
Organism Enterobacter hormaechei subsp. xiangfangensis strain Ec61

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag HLA96_RS16835 Protein ID WP_015571638.1
Coordinates 3570714..3571013 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0F0ZCT3
Locus tag HLA96_RS16840 Protein ID WP_022651701.1
Coordinates 3571024..3571371 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLA96_RS16825 3567091..3569283 + 2193 WP_137041666.1 type I secretion system permease/ATPase -
HLA96_RS16830 3569303..3570463 + 1161 WP_086581339.1 HlyD family efflux transporter periplasmic adaptor subunit -
HLA96_RS16835 3570714..3571013 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HLA96_RS16840 3571024..3571371 + 348 WP_022651701.1 HigA family addiction module antidote protein Antitoxin
HLA96_RS16845 3571447..3572628 - 1182 WP_023314694.1 PLP-dependent aminotransferase family protein -
HLA96_RS16850 3572648..3573535 - 888 WP_022651703.1 LysR family transcriptional regulator -
HLA96_RS16855 3573633..3574235 + 603 WP_032660049.1 short chain dehydrogenase -
HLA96_RS16860 3574232..3574963 - 732 WP_032660054.1 class I SAM-dependent methyltransferase -
HLA96_RS16865 3575038..3576036 - 999 WP_160858545.1 transketolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T156153 WP_015571638.1 NZ_CP053103:3570714-3571013 [Enterobacter hormaechei subsp. xiangfangensis]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T156153 NZ_CP068829:737452-737748 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCTGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13096.09 Da        Isoelectric Point: 9.5932

>AT156153 WP_022651701.1 NZ_CP053103:3571024-3571371 [Enterobacter hormaechei subsp. xiangfangensis]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVA

Download         Length: 348 bp

>AT156153 NZ_CP068829:737750-738145 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F0ZCT3

References