Detailed information of TA system TA156132

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrG-as-bsrH/-
• Location: 2385953..2386243
• Replicon: chromosome
• Accession: NZ_CP053102
• Organism: Bacillus subtilis subsp. subtilis str. 168

Toxin (Protein)

• Gene name: bsrG
• Uniprot ID: L8EAY0
• Locus tag: HIR77_RS12445
• Protein ID: WP_009967548.1
• Coordinates: 2385953..2386069 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	as-bsrH
Locus tag	-
Coordinates	2386064..2386243 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIR77_RS12410	2381880..2382050	-	171	WP_009967544.1	bacteriocin sublancin-168	-
HIR77_RS12415	2382347..2382664	-	318	WP_009967545.1	sublancin immunity protein SunI	-
HIR77_RS12420	2382766..2384016	-	1251	WP_004398504.1	UV-damage repair protein uvrX	-
HIR77_RS12425	2384009..2384341	-	333	WP_004398710.1	YolD-like family protein	-
HIR77_RS12430	2384515..2384850	+	336	WP_004399073.1	hypothetical protein	-
HIR77_RS12435	2384893..2385249	-	357	WP_004398595.1	hypothetical protein	-
HIR77_RS12440	2385255..2385722	-	468	WP_003246138.1	YolA family protein	-
HIR77_RS12445	2385953..2386069	+	117	WP_009967548.1	type I toxin-antitoxin system toxin BsrG	Toxin
-	2386064..2386243	-	180	NuclAT_1	-	Antitoxin
-	2386064..2386243	-	180	NuclAT_1	-	Antitoxin
-	2386064..2386243	-	180	NuclAT_1	-	Antitoxin
-	2386064..2386243	-	180	NuclAT_1	-	Antitoxin
HIR77_RS12450	2386348..2386881	-	534	WP_004399156.1	GNAT family N-acetyltransferase	-
HIR77_RS12455	2386917..2387495	-	579	WP_004399123.1	SMI1/KNR4 family protein	-
HIR77_RS12460	2387559..2388056	-	498	WP_003246188.1	SMI1/KNR4 family protein	-
HIR77_RS12465	2388065..2389780	-	1716	WP_004398855.1	ribonuclease YeeF family protein	-
HIR77_RS12470	2389880..2390437	-	558	WP_003246042.1	SMI1/KNR4 family protein	-
HIR77_RS12475	2390521..2390706	+	186	WP_003245951.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2141170..2398767	257597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-38)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4336.39 Da
• Isoelectric Point: 10.1022

>T156132 WP_009967548.1 NZ_CP053102:2385953-2386069 [Bacillus subtilis subsp. subtilis str. 168]
MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK

Nucleotide

• Length: 117 bp

>T156132 NZ_CP068828:c883189-882812 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGACTACTGGGCCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 180 bp

>AT156132 NZ_CP053102:c2386243-2386064 [Bacillus subtilis subsp. subtilis str. 168]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7Z7EVU9

Antitoxin