Detailed information of TA system TA156087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8322..8973
• Replicon: plasmid unnamed1
• Accession: NZ_CP053099
• Organism: Acinetobacter baumannii ATCC 17978

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V2UF49
• Locus tag: HKO16_RS19150
• Protein ID: WP_000269909.1
• Coordinates: 8322..8678 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HKO16_RS19155
• Protein ID: WP_001140621.1
• Coordinates: 8671..8973 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKO16_RS19120	3397..3786	+	390	WP_000078281.1	tautomerase family protein	-
HKO16_RS19125	3969..4112	-	144	WP_001125249.1	hypothetical protein	-
HKO16_RS19130	4133..4687	-	555	WP_001096621.1	plasmid replication DNA-binding protein	-
HKO16_RS19135	4680..5636	-	957	WP_000786840.1	replication initiation protein RepM	-
HKO16_RS19140	6601..6969	+	369	WP_000139283.1	LysR family transcriptional regulator	-
HKO16_RS19145	7171..7308	+	138	Protein_8	transposase	-
HKO16_RS19150	8322..8678	+	357	WP_000269909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKO16_RS19155	8671..8973	+	303	WP_001140621.1	XRE family transcriptional regulator	Antitoxin
HKO16_RS19160	8966..9175	+	210	WP_000069472.1	hypothetical protein	-
HKO16_RS19165	9313..10443	+	1131	WP_000085720.1	DUF262 domain-containing protein	-
HKO16_RS19170	10450..11163	+	714	WP_061855755.1	hypothetical protein	-
HKO16_RS19175	11361..11972	+	612	WP_000571899.1	recombinase family protein	-
HKO16_RS19180	12188..12706	+	519	WP_000447196.1	sel1 repeat family protein	-
HKO16_RS19185	12745..13257	+	513	WP_001089572.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..13409	13409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13515.49 Da
• Isoelectric Point: 6.4631

>T156087 WP_000269909.1 NZ_CP053099:8322-8678 [Acinetobacter baumannii ATCC 17978]
MWTVITTDLFNEWLVQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T156087 NZ_CP068826:c4042856-4042458 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11179.02 Da
• Isoelectric Point: 7.3198

>AT156087 WP_001140621.1 NZ_CP053099:8671-8973 [Acinetobacter baumannii ATCC 17978]
MAKTLQELLASRSPESQARIQKMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT156087 NZ_CP068826:c4043152-4042859 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: V2UF49