Detailed information of TA system TA156083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 30600..31116
• Replicon: plasmid pCLP-Y5-2
• Accession: NZ_CP053095
• Organism: Lacticaseibacillus paracasei strain CLP-Y5

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: HLG73_RS15545
• Protein ID: WP_003573774.1
• Coordinates: 30835..31116 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: HLG73_RS15540
• Protein ID: WP_016363783.1
• Coordinates: 30600..30842 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLG73_RS15525 (HLG73_15740)	27949..28653	+	705	WP_079323062.1	response regulator transcription factor	-
HLG73_RS15530 (HLG73_15745)	28818..29738	+	921	WP_273634851.1	IS30 family transposase	-
HLG73_RS15535 (HLG73_15750)	29761..30330	+	570	Protein_28	transposase	-
HLG73_RS15540 (HLG73_15755)	30600..30842	+	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HLG73_RS15545 (HLG73_15760)	30835..31116	+	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HLG73_RS15550 (HLG73_15765)	31253..31903	+	651	WP_003586220.1	recombinase family protein	-
HLG73_RS15555 (HLG73_15770)	31909..32193	+	285	WP_016383626.1	BRCT domain-containing protein	-
HLG73_RS15560 (HLG73_15775)	32585..32716	+	132	Protein_33	helix-turn-helix domain-containing protein	-
HLG73_RS15565 (HLG73_15780)	32775..33731	-	957	WP_003569652.1	LacI family DNA-binding transcriptional regulator	-
HLG73_RS15570 (HLG73_15785)	33915..34772	-	858	WP_003569650.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59366	59366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T156083 WP_003573774.1 NZ_CP053095:30835-31116 [Lacticaseibacillus paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T156083 NZ_CP068826:3171379-3171873 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT156083 WP_016363783.1 NZ_CP053095:30600-30842 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT156083 NZ_CP068826:3171089-3171382 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7