Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3537446..3538116 Replicon chromosome
Accession NZ_CP053092
Organism Mycobacterium tuberculosis strain 4860

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag HGO90_RS16695 Protein ID WP_003899954.1
Coordinates 3537446..3537790 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag HGO90_RS16700 Protein ID WP_003899955.1
Coordinates 3537787..3538116 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGO90_RS16665 3532519..3533379 + 861 WP_003416628.1 alpha/beta hydrolase -
HGO90_RS16670 3533354..3533869 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
HGO90_RS16675 3534109..3534402 + 294 WP_003416635.1 hypothetical protein -
HGO90_RS16680 3534690..3535979 + 1290 WP_003906996.1 ATP-binding protein -
HGO90_RS16685 3536326..3536760 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
HGO90_RS16690 3536763..3537215 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HGO90_RS16695 3537446..3537790 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HGO90_RS16700 3537787..3538116 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
HGO90_RS16705 3538654..3539001 + 348 WP_003899956.1 DUF2384 domain-containing protein -
HGO90_RS16710 3538998..3539618 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
HGO90_RS16715 3539778..3541043 - 1266 WP_003902423.1 hypothetical protein -
HGO90_RS16720 3541211..3541420 + 210 WP_003416778.1 hypothetical protein -
HGO90_RS16725 3541667..3542701 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T156075 WP_003899954.1 NZ_CP053092:3537446-3537790 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T156075 NZ_CP068826:978153-978560 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT156075 WP_003899955.1 NZ_CP053092:3537787-3538116 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT156075 NZ_CP068826:977906-978172 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References