Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2249338..2250257 Replicon chromosome
Accession NZ_CP053092
Organism Mycobacterium tuberculosis strain 4860

Toxin (Protein)


Gene name HigB2 Uniprot ID A0A654U351
Locus tag HGO90_RS10520 Protein ID WP_003906719.1
Coordinates 2249652..2250257 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag HGO90_RS10515 Protein ID WP_003410124.1
Coordinates 2249338..2249643 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGO90_RS10485 2244349..2245605 - 1257 WP_003410095.1 HNH endonuclease -
HGO90_RS10490 2245959..2246534 + 576 WP_003410100.1 hypothetical protein -
HGO90_RS10495 2246531..2247571 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
HGO90_RS10500 2247813..2248532 + 720 WP_003410108.1 DUF433 domain-containing protein -
HGO90_RS10505 2248522..2248938 + 417 WP_003410114.1 hypothetical protein -
HGO90_RS10510 2248954..2249253 - 300 WP_003410120.1 hypothetical protein -
HGO90_RS10515 2249338..2249643 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
HGO90_RS10520 2249652..2250257 - 606 WP_003906719.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HGO90_RS10525 2250282..2250641 - 360 WP_003410131.1 hypothetical protein -
HGO90_RS10530 2250801..2251478 - 678 WP_003900450.1 hypothetical protein -
HGO90_RS10535 2251430..2251666 - 237 WP_003410133.1 hypothetical protein -
HGO90_RS10540 2251755..2253016 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
HGO90_RS10545 2253231..2254127 - 897 WP_003906720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22716.91 Da        Isoelectric Point: 7.3073

>T156057 WP_003906719.1 NZ_CP053092:c2250257-2249652 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T156057 NZ_CP068825:c2084304-2084197 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT156057 WP_003410124.1 NZ_CP053092:c2249643-2249338 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT156057 NZ_CP068825:2084352-2084418 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654U351

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References