Detailed information of TA system TA156002

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30038..30307
• Replicon: plasmid pHB37-2
• Accession: NZ_CP053082
• Organism: Escherichia coli strain HB37

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HKW69_RS26300
• Protein ID: WP_001312861.1
• Coordinates: 30149..30307 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30038..30103 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKW69_RS26270	25748..26275	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HKW69_RS26275	26333..26566	+	234	WP_000006003.1	DUF905 family protein	-
HKW69_RS26280	26627..28650	+	2024	Protein_37	ParB/RepB/Spo0J family partition protein	-
HKW69_RS26285	28719..29153	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HKW69_RS26290	29150..29869	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	29881..30105	+	225	NuclAT_0	-	-
-	29881..30105	+	225	NuclAT_0	-	-
-	29881..30105	+	225	NuclAT_0	-	-
-	29881..30105	+	225	NuclAT_0	-	-
HKW69_RS26295	29890..30069	-	180	WP_001309233.1	hypothetical protein	-
-	30038..30103	+	66	-	-	Antitoxin
HKW69_RS26300	30149..30307	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HKW69_RS26960	30545..30922	-	378	Protein_42	hypothetical protein	-
HKW69_RS26310	31222..31518	+	297	WP_001272251.1	hypothetical protein	-
HKW69_RS26315	31629..32450	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HKW69_RS26320	32747..33394	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HKW69_RS26325	33671..34054	+	384	WP_000124981.1	relaxosome protein TraM	-
HKW69_RS26330	34245..34931	+	687	WP_015059009.1	PAS domain-containing protein	-
HKW69_RS26335	35025..35252	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB	-	1..71222	71222

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T156002 WP_001312861.1 NZ_CP053082:30149-30307 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T156002 NZ_CP068823:350712-350987 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT156002 NZ_CP053082:30038-30103 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin