Detailed information of TA system TA155994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5073675..5074277
• Replicon: chromosome
• Accession: NZ_CP053080
• Organism: Escherichia coli strain HB37

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HKW69_RS24335
• Protein ID: WP_000897305.1
• Coordinates: 5073966..5074277 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HKW69_RS24330
• Protein ID: WP_000356397.1
• Coordinates: 5073675..5073965 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKW69_RS24310	5070177..5071079	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HKW69_RS24315	5071076..5071711	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HKW69_RS24320	5071708..5072637	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HKW69_RS24325	5072852..5073070	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
HKW69_RS24330	5073675..5073965	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HKW69_RS24335	5073966..5074277	-	312	WP_000897305.1	hypothetical protein	Toxin
HKW69_RS24340	5074506..5075414	+	909	WP_001298596.1	alpha/beta hydrolase	-
HKW69_RS24345	5075478..5076419	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HKW69_RS24350	5076464..5076901	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HKW69_RS24355	5076898..5077770	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HKW69_RS24360	5077764..5078363	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T155994 WP_000897305.1 NZ_CP053080:c5074277-5073966 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155994 NZ_CP068822:c4676368-4676195 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155994 WP_000356397.1 NZ_CP053080:c5073965-5073675 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155994 NZ_CP068822:c4676692-4676396 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7