Detailed information of TA system TA155984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4069686..4070380
• Replicon: chromosome
• Accession: NZ_CP053080
• Organism: Escherichia coli strain HB37

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HKW69_RS19625
• Protein ID: WP_001263500.1
• Coordinates: 4069686..4070084 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HKW69_RS19630
• Protein ID: WP_000554758.1
• Coordinates: 4070087..4070380 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKW69_RS19600	4065051..4065509	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
HKW69_RS19605	4065770..4067227	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HKW69_RS19610	4067284..4067898	-	615	WP_000602124.1	peptide chain release factor H	-
HKW69_RS19615	4067895..4069034	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
HKW69_RS19620	4069224..4069676	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HKW69_RS19625	4069686..4070084	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HKW69_RS19630	4070087..4070380	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HKW69_RS19635	4070432..4071487	-	1056	WP_001226168.1	DNA polymerase IV	-
HKW69_RS19640	4071558..4072343	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
HKW69_RS19645	4072315..4074027	+	1713	Protein_3844	flagellar biosynthesis protein FlhA	-
HKW69_RS19650	4074055..4074264	+	210	WP_071778446.1	hypothetical protein	-
HKW69_RS19655	4074347..4074844	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	4069686..4090040	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T155984 WP_001263500.1 NZ_CP053080:c4070084-4069686 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T155984 NZ_CP068822:c3171116-3170733 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT155984 WP_000554758.1 NZ_CP053080:c4070380-4070087 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT155984 NZ_CP068822:c3171577-3171197 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCACCGGAATAG

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3