Detailed information of TA system TA155964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 750320..751119
• Replicon: chromosome
• Accession: NZ_CP053080
• Organism: Escherichia coli strain HB37

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: HKW69_RS03725
• Protein ID: WP_000347252.1
• Coordinates: 750320..750784 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: HKW69_RS03730
• Protein ID: WP_001296435.1
• Coordinates: 750784..751119 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKW69_RS03695	745321..745755	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
HKW69_RS03700	745773..746651	-	879	WP_023152018.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HKW69_RS03705	746641..747420	-	780	WP_170171932.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HKW69_RS03710	747431..747904	-	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HKW69_RS03715	747927..749207	-	1281	WP_000681930.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HKW69_RS03720	749456..750265	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HKW69_RS03725	750320..750784	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HKW69_RS03730	750784..751119	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HKW69_RS03735	751268..752839	-	1572	WP_001273939.1	galactarate dehydratase	-
HKW69_RS03740	753214..754548	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
HKW69_RS03745	754564..755334	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T155964 WP_000347252.1 NZ_CP053080:c750784-750320 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T155964 NZ_CP068821:c4052042-4051644 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT155964 WP_001296435.1 NZ_CP053080:c751119-750784 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT155964 NZ_CP068821:c4052338-4052045 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5