Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2562373..2562935 Replicon chromosome
Accession NZ_CP053079
Organism Escherichia coli strain K56-43-un

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HK408_RS12055 Protein ID WP_000605675.1
Coordinates 2562373..2562651 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HK408_RS12060 Protein ID WP_000781370.1
Coordinates 2562651..2562935 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HK408_RS12030 2557959..2558390 - 432 WP_021522095.1 peroxiredoxin OsmC -
HK408_RS12035 2558735..2558950 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HK408_RS12040 2559052..2559189 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HK408_RS12045 2559346..2561043 + 1698 WP_021522093.1 malate dehydrogenase -
HK408_RS12050 2561177..2562187 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HK408_RS12055 2562373..2562651 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HK408_RS12060 2562651..2562935 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HK408_RS12065 2563164..2563817 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HK408_RS12070 2563810..2564694 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HK408_RS12075 2564707..2567754 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T155952 WP_000605675.1 NZ_CP053079:2562373-2562651 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T155952 NZ_CP068821:1977338-1977592 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT155952 WP_000781370.1 NZ_CP053079:2562651-2562935 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT155952 NZ_CP068821:1977090-1977341 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References