Detailed information of TA system TA155941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1265767..1266494
• Replicon: chromosome
• Accession: NZ_CP053079
• Organism: Escherichia coli strain K56-43-un

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: HK408_RS06025
• Protein ID: WP_000547563.1
• Coordinates: 1265767..1266078 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HK408_RS06030
• Protein ID: WP_000126304.1
• Coordinates: 1266075..1266494 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HK408_RS05995	1260911..1262620	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
HK408_RS06000	1262630..1263172	+	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
HK408_RS06005	1263172..1263939	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
HK408_RS06010	1263936..1264346	+	411	WP_001291913.1	formate hydrogenlyase assembly protein HycH	-
HK408_RS06015	1264339..1264806	+	468	WP_021522200.1	hydrogenase maturation peptidase HycI	-
HK408_RS06020	1264849..1265604	+	756	WP_001314074.1	hypothetical protein	-
HK408_RS06025	1265767..1266078	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
HK408_RS06030	1266075..1266494	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
HK408_RS06035	1266586..1267014	-	429	WP_021522199.1	hypothetical protein	-
HK408_RS06040	1267399..1267926	+	528	WP_001078777.1	electron transport protein HydN	-
HK408_RS06045	1268079..1270331	+	2253	WP_021522198.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T155941 WP_000547563.1 NZ_CP053079:1265767-1266078 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T155941 NZ_CP068821:c72957-72580 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCATTTGTCCCCGCTCTCAGTTAATAAACAGTATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT155941 WP_000126304.1 NZ_CP053079:1266075-1266494 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT155941 NZ_CP068821:c73415-73047 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCAGATGCATACCACCTGGACCAGGCCTTTGCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22