Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 631484..632283 Replicon chromosome
Accession NZ_CP053079
Organism Escherichia coli strain K56-43-un

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HK408_RS03050 Protein ID WP_000347251.1
Coordinates 631484..631948 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HK408_RS03055 Protein ID WP_001296435.1
Coordinates 631948..632283 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HK408_RS03020 626485..626919 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HK408_RS03025 626937..627815 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HK408_RS03030 627805..628584 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HK408_RS03035 628595..629068 - 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
HK408_RS03040 629091..630371 - 1281 WP_000681931.1 tagatose-bisphosphate aldolase subunit KbaZ -
HK408_RS03045 630620..631429 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HK408_RS03050 631484..631948 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HK408_RS03055 631948..632283 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HK408_RS03060 632432..634003 - 1572 WP_001539800.1 galactarate dehydratase -
HK408_RS03065 634378..635712 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
HK408_RS03070 635728..636498 + 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T155937 WP_000347251.1 NZ_CP053079:c631948-631484 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T155937 NZ_CP068820:c4197777-4197400 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT155937 WP_001296435.1 NZ_CP053079:c632283-631948 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT155937 NZ_CP068820:c4198235-4197867 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGACGACAATCGCGGCCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCACGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References