Detailed information of TA system TA155935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 4085..4609
• Replicon: plasmid pSA01-tet
• Accession: NZ_CP053076
• Organism: Staphylococcus aureus strain SA01

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: HK402_RS14515
• Protein ID: WP_170172424.1
• Coordinates: 4085..4351 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: HK402_RS14520
• Protein ID: WP_069822313.1
• Coordinates: 4352..4609 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HK402_RS14495	1..960	+	960	WP_170172421.1	replication initiator protein A	-
HK402_RS14500	953..1279	+	327	WP_170172430.1	protein rep	-
HK402_RS14505	1923..2438	+	516	WP_170172422.1	MarR family transcriptional regulator	-
HK402_RS14510	2435..2794	+	360	WP_170172423.1	rhodanese-like domain-containing protein	-
HK402_RS14515	4085..4351	-	267	WP_170172424.1	Txe/YoeB family addiction module toxin	Toxin
HK402_RS14520	4352..4609	-	258	WP_069822313.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HK402_RS14525	4812..4904	+	93	WP_023016171.1	type I toxin-antitoxin system Fst family toxin	-
HK402_RS14530	5064..5201	-	138	WP_170172425.1	hypothetical protein	-
HK402_RS14535	5430..6413	+	984	WP_000336291.1	protein rep	-
HK402_RS14540	6573..7343	+	771	WP_001795128.1	aminoglycoside O-nucleotidyltransferase ANT(4')-Ia	-
HK402_RS14545	7669..8343	+	675	WP_113589581.1	IS6 family transposase	-
HK402_RS14550	8423..9157	-	735	WP_014158488.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(C)	-
HK402_RS14555	9215..9274	-	60	WP_002497446.1	23S rRNA methylase leader peptide ErmCL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aadD / erm(C) / aac(6')-aph(2'')	-	1..25664	25664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (23-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10543.95 Da
• Isoelectric Point: 9.6414

>T155935 WP_170172424.1 NZ_CP053076:c4351-4085 [Staphylococcus aureus]
MDNYSISIKNSAKSDLKKIKQSQLKTQFLKIVDTLKEDPYFPNQSFEKLQPKHLGRYSRRINHQHRVVYTVDEDDKEVFI
LSAWSHYE

Nucleotide

• Length: 267 bp

>T155935 NZ_CP068820:3579355-3579462 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9880.86 Da
• Isoelectric Point: 4.2797

>AT155935 WP_069822313.1 NZ_CP053076:c4609-4352 [Staphylococcus aureus]
MNIKTPTNARKEFYELLKQVNDSHEPVIISSSKSENNAVIISQKDWDSIQETLYLETTGTMDKVREREKDNSGYTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT155935 NZ_CP068820:c3579306-3579243 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT