Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4746433..4747142 Replicon chromosome
Accession NZ_CP053068
Organism Mycobacterium avium subsp. paratuberculosis strain DSM 44135

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag MAP44135_RS22290 Protein ID WP_003873387.1
Coordinates 4746738..4747142 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag MAP44135_RS22285 Protein ID WP_003873388.1
Coordinates 4746433..4746741 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAP44135_RS22270 4742318..4742620 + 303 WP_003873391.1 co-chaperone GroES -
MAP44135_RS22275 4742709..4744325 + 1617 WP_003873390.1 chaperonin GroEL -
MAP44135_RS22280 4744420..4745946 + 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
MAP44135_RS22285 4746433..4746741 - 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
MAP44135_RS22290 4746738..4747142 - 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MAP44135_RS22295 4747382..4748074 + 693 WP_010950286.1 DUF4352 domain-containing protein -
MAP44135_RS22300 4748129..4749328 + 1200 WP_010950287.1 FAD-dependent oxidoreductase -
MAP44135_RS22305 4749485..4750249 - 765 WP_003874401.1 superoxide dismutase family protein -
MAP44135_RS22310 4750292..4750600 - 309 WP_003874400.1 WhiB family transcriptional regulator -
MAP44135_RS22315 4750998..4751828 + 831 WP_003874399.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T155899 WP_003873387.1 NZ_CP053068:c4747142-4746738 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T155899 NZ_CP068819:2882684-2882791 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT155899 WP_003873388.1 NZ_CP053068:c4746741-4746433 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT155899 NZ_CP068819:c2882637-2882571 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References