Detailed information of TA system TA155870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1200965..1201644
• Replicon: chromosome
• Accession: NZ_CP053045
• Organism: Escherichia fergusonii strain HNCF11W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HG538_RS05805
• Protein ID: WP_002431667.1
• Coordinates: 1200965..1201267 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HG538_RS05810
• Protein ID: WP_000806440.1
• Coordinates: 1201303..1201644 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG538_RS05780	1196341..1197993	+	1653	WP_000670589.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HG538_RS05785	1198031..1198534	-	504	WP_000667002.1	hypothetical protein	-
HG538_RS05790	1198531..1199331	-	801	WP_002431995.1	hypothetical protein	-
HG538_RS05795	1199355..1199834	-	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HG538_RS05800	1200038..1200832	-	795	WP_000365155.1	TraB/GumN family protein	-
HG538_RS05805	1200965..1201267	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HG538_RS05810	1201303..1201644	+	342	WP_000806440.1	HigA family addiction module antidote protein	Antitoxin
HG538_RS05815	1201758..1204262	-	2505	WP_000083946.1	copper-exporting P-type ATPase CopA	-
HG538_RS05820	1204569..1205858	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T155870 WP_002431667.1 NZ_CP053045:1200965-1201267 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T155870 NZ_CP068818:c2690818-2690642 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT155870 WP_000806440.1 NZ_CP053045:1201303-1201644 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT155870 NZ_CP068818:c2690596-2690180 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8