Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3861984..3862581 Replicon chromosome
Accession NZ_CP053035
Organism Klebsiella pneumoniae strain P35

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HKJ75_RS18805 Protein ID WP_004142563.1
Coordinates 3862264..3862581 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HKJ75_RS18800 Protein ID WP_004142561.1
Coordinates 3861984..3862271 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ75_RS18770 3858075..3858323 + 249 WP_020325243.1 DUF1158 domain-containing protein -
HKJ75_RS18775 3858341..3858682 - 342 WP_117077680.1 RamA family antibiotic efflux transcriptional regulator -
HKJ75_RS18780 3858713..3859828 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
HKJ75_RS18785 3860008..3860578 + 571 Protein_3683 TetR/AcrR family transcriptional regulator -
HKJ75_RS18790 3860578..3860946 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
HKJ75_RS18795 3861066..3861719 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HKJ75_RS18800 3861984..3862271 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HKJ75_RS18805 3862264..3862581 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HKJ75_RS18810 3862766..3863809 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
HKJ75_RS18815 3864479..3865345 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HKJ75_RS18820 3865454..3866881 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T155842 WP_004142563.1 NZ_CP053035:c3862581-3862264 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T155842 NZ_CP068817:2621619-2621897 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT155842 WP_004142561.1 NZ_CP053035:c3862271-3861984 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT155842 NZ_CP068817:2621897-2622181 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References